Salmon Calcitonin Acetate
Product Description
Salmon Calcitonin Acetate
Source: Synthetic
Background: Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, paget's disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity.
Description: Calcitonin Acetate (Salmon) is a synthetic polypeptide of 32 amino acids in the same linear sequence that is found in clacitonin of salmon origin. MW=3432
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Solubility: The lyophilized Calcitonin is very soluble in water and most aqueous buffers below and above the isoelectric point.
Stability: Lyophilized Calcitonin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Calcitonin should be stored at 4 C between 2-7 days and for future use below -18 C. Please avoid freeze-thaw cycles.
Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: Cys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Gly-Thr-Pro-NH2
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ g (IEU/ g) of Calcitonin.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC.
Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
