One of the original Good’s buffers, PIPES (Piperazine-N,N′-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH, making it useful in cell culture work. PIPES has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions.
