Product Description
Product Description
Tetracycline is an antibiotic that possesses bacteriostatic activity against most medically relevant aerobic and anaerobic Gram-positive and Gram-negative bacteria. Tetracycline blocks the ability of bacteria to make proteins by binding to the 30S subunit of microbial ribosomes. The attachment of charged aminoacyl-tRNA to the A site on the ribosome becomes blocked by tetracycline, preventing introduction of new amino acids to the nascent peptide chain.
BSA (bovine serum albumin) is conjugated to tetracycline (1: 138). The amine in tetracycline is directly linked to carboxyl group(s) in BSA. Tetracycline has a molecular weight of 480.9 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS at a pH of 7.4.
Conjugated BSA-tetracycline is used to recognize tetracycline-specific antibodies by ELISA and lateral flow based immunoassay.
