Ampicillin HP
Product Description
HP-Ampicillin eliminates the problem of satellite or background colonies, even after a prolonged incubation (2-4 days) or the plating of competent cells at 20 fold higher density. β-Lactamase secreted into the medium from ampicillin-resistant transformants rapidly inactivates regular ampicillin in the region surrounding the colonies. Thus plating cells at high density or incubating them for prolonged periods of time results in the appearance of ampicillin sensitive satellite colonies. This problem is ameliorated, but not completely eliminated, by using a high concentration of ampicillin, carbenicillin, or methicillin. HP-Ampicillin is a stronger inhibitor of B-Lactamase than regular ampicillin and, therefore, supports the growth of only ampicillin resistant colonies.
Kit Components
1. HP-Ampicillin (1000X) - 5x1 ml
Storage Conditions
- HP-Ampicillin is stored at - 20*C and is stable for six months.
Applications
1.As an antibiotic in growing plasmid DNA in liquid cultures or plates.
2.As an antibiotic in tissue culture.
Advantages
1.Eliminates the growth of non-specific satellite colonies.
2.Plates can be incubated for longer period of time at 37ºC.
- For in vitro lab use only. Not for human use or consumption.
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
- Appearance: Liquid
