Dextran Sulfate

Product Description

A glycosaminoglycan that acts as a precipitant for high density lipo- proteins (HDLs). Used in hybridization protocols where it effectively increases the probe concentration accelerating the rate of annealing.

Specifications

• Ultra Pure Grade
• Moisture: <10.0%
• Heavy metals (as Pb): <0.005%
• Iron: <0.001%

Increasing the hybridization rate of nucleic acids - the presence of 10% dextran sulfate increases the rate of reannealing of DNA in solution by about 10 times. In the presence of 10% dextran sulfate the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids may increase up to 100 times.

Immunologically relevant activities

• changes in thymocyte reactivity to lectins
• enhancement/suppression of cell-mediated immune responses.
• enhancement and suppression of humoral immunity
• polyclonal activation of B-lymphocytes, stimulating even immature B cells6
• inhibition of blood coagulation and platelet aggregation
  1. Blitstein-Willinger, E., Schutz, G. and Diamanststein, T., 'Changes in thymocyte reactivity to lectins by B-cell mitogens of the type of sulphated polyanions.' Immunology, v. 30, 529-533 (1976).
  2. Bradfield, J.W.B., Souhami, R.L., Addison, J.E., 'The mechanism of the adjuvant action of dextran sulphate.' Immunology, v. 26, 383-386 (1974).
  3. Diamantstein, T., et al., 'Stimulation of humoral antibody formation by polyanions. II. The influence of sulphate esters of polymers on the immune response on mice.' Eur. J. Immunol., v. 1, 340-343 (1971).
  4. Diamantstein, T., et al., 'Suppression of the primary immune response in vivo to sheep red blood cells by B-cell mitogens.' Immunology, v. 30, 401-405 (1976).
  5. Finley, P.R., et al., 'Cholesterol in high-density lipoprotein: Use of Mg+2/dextran sulphate in its enzymatic measurement.' Clin. Chem., v. 24, 931-933 (1978).
  6. Granstrom, M., et al., 'The polyclonal B-cell activator Dextran sulphate induces formation of colony stimulating activity.' Scand. J. Immunol., v. 7, 277-284 (1978).
  7. Laderman, L., Kawasaki, E.S and Szabo, P., 'The rate of nucleic acid annealing to cytological preparation is increased in the presence of dextran sulphate.' Anal. Biochem., v. 117, 158-163 (1981).
  8. Lambin, P. and Burstein, M., 'Isolation of a beta2-glycoprotein from human serum after precipitation with dextran sulphate and manganese chloride.' Biochimie, v. 64, 1065-1071 (1982).
  9. Maniatis, T., Fritsch, E. and Sambrook, F. (eds.), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory (1982).
  10. McCarthey, R.E. and Babcock, G.F., 'Simultaneous stimulation and suppression of two different indicators of the cell-mediated immune response by the immunoregulator dextran sulphate.' Immunology, v. 34, 827-929 (1978).
  11. Nakajima, K., et al., 'Effects of dextran sulphate on blood coagulation and fibrinolysis in spontaneously hypertensive rats.' Adv. Inflamm. Res., v. 1, 331-337 (1979).
  12. Ricketts, C.R., 'Dextran Sulphate - A synthetic analogue of Heparin.' Biochemistry, v. 51, 129-133 (1952).
  13. Ricketts, C.R., 'A basic derivative of dextran and its interaction with serum albumin.' Biochem. J., v. 76, 117-120 (1960).
  14. Warnick, G.R., et al., 'HDL cholesterol quantitation by phosphotungstate - Mg2+ and by dextran sulphate - Mn2+ - polyethylene glycol precipitation, both with enzymatic cholesterol assay compared with the lipid research method.' Amer. J. Clin. Pathol., v. 78, 718-723 (1982).
  15. Wetmur, J.G., 'Acceleration of DNA renaturation rates.' Biopolymers, v. 14, 2517-2524 (1975).
  16. Wahl, G.M., et al., 'Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization with dextran sulfate.' Proc. Natl. Acad. Sci. USA, v. 76, 3683 (1979).

Precipitation of LDL and VLDL lipoproteins - in the presence of Mg ions, dextran sulfate precipitates low-density lipoproteins from human serum. May be used as an adjuvant

References

Appearance: Solid PH Value: pH (1%, water): 6.4-8.0 RTECS Number: HH9290000

Technical Specifications

Composition

Technical Documentation