Low potential for eliciting an immune response, ensuring that the primary response is to the antigen-carrier protein conjugate and not against a determinant on the crosslinker.
Less immunogenic than SMCC
Reactive groups: NHS ester and maleimide
Reactive toward: amino and sulfhydryl groups
References:
Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
Fujiwara, K., et al. (1988). Sandwich enzyme immunoassay of tumor-associated antigen sialosylated Lewis (x) using ß-D-galactosidase coupled to a monoclonal antibody of IgM isotype. J. Immunol. Meth. 112, 77-83.
