Product Description
Product Description
Penicillin is a group of β-lactam antibiotics and were the first medication to be effective against bacterial infections caused by staphylococci and streptococci. Penicillin inhibits the formation of peptidoglycan cross-links in the bacterial cell wall through binding of the β-lactam ring of penicillin to the enzyme DD-transpeptidase. DD-transpeptidase cannot catalyze formation of these cross-links, and an imbalance between cell wall production and degradation develops, causing the cell to die. Many types of bacteria have developed resistance from extensive use.
BSA (bovine serum albumin) is conjugated to penicillin (1: 199). The carboxyl group in penicillin is directly linked to an amine group in BSA. Penicillin has a molecular weight of 334.4 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS at a pH of 7.4.
Conjugated BSA-penicillin is used to recognize penicillin-specific antibodies by ELISA and lateral flow based immunoassay.
