Product Description
Product Description
Streptomycin is an antibiotic used to treat Gram-positive and Gram-negative bacterial infections. Streptomycin is a protein synthesis inhibitor that binds to the small 16S rRNA of the 30S subunit of the bacterial ribosome, interfering with the binding of formyl-methionyl-tRNA to the 30S subunit. The binding of the molecule to the 30S subunit interferes with 50S subunit association with the mRNA strand, resulting in a frameshift mutation and defective protein synthesis which leads to cell death.
BSA (bovine serum albumin) is conjugated to streptomycin (1: 91). One or both of the two amine groups in streptomycin are directly linked to carboxyl group(s) in BSA. Streptomycin has a molecular weight of 728.7 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS at a pH of 7.4.
Conjugated BSA-streptomycin is used to recognize streptomycin-specific antibodies by ELISA and lateral flow based immunoassay.
