TMB Stop Reagent 450 nm
Product Description
TMB Stop Reagent
Stop reagent is 0.16M sulfuric acid used to stop the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB) reaction. Horseradish peroxidase (HRP) enzyme conjugates react with TMB and peroxide to produce a blue byproduct having maximum absorbance at 605nm. Adding Stop reagent changes it to yellow color with absorption maximum at 450 nm and stabilizes the color development.
